The fee of E-to-A conversion and the proportions of the different A varieties synthesized in vitro had been just like these noticed for the tissues in vivo at comparable times. These results recommend that the post translational processing in vitro was much like that in vivo. The data had been in keeping with the likelihood that the rise in synthesis of A types occurred both in cells that had previously synthesized E types or in a definite inhabitants of cells that already synthesized A varieties. In pulse-chase experiments, the E type of N-CAM that was synthesized in the course of the first day after explantation endured as E kind for a quantity of days, at instances when newly synthesized N-CAM was predominantly in A varieties.
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During normal development, the neural cell adhesion molecule N-CAM modifications on the cell-surface from a sialic acid-rich embryonic, or E type, to several adult, or A varieties that have much less sialic acid (E-to-A conversion). To examine the cellular and molecular mechanisms that underlie these changes, we have established situations underneath which E-to-A conversion occurs in cultured explants of central nervous system tissues. Mouse cerebellum, chick spinal twine, and chick retina that express the E type of N-CAM have been dissected and cultured on collagen gels. After 3-6 d in culture, elevated proportions of A forms have been synthesized, as revealed by particular immunoprecipitation and immunoblotting.
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